Part:BBa_K2302008:Design

PelB+CALB+His

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1023
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 25
  Illegal NgoMIV site found at 51
  Illegal NgoMIV site found at 744
  Illegal NgoMIV site found at 854
  Illegal AgeI site found at 546
  Illegal AgeI site found at 804
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

the plasmid will be transform into E. coli DE3 for protein expression.

Source

these genes are subcloned from the genome of E. coli DE3.